Beckman multimek 96 manual4/30/2023 ![]() ![]() ![]() The OD method was the DNA quantification method most concordant with the reference sample among the three methods evaluated. The effects of manual and robotic laboratory sample handling procedures on the estimates of DNA concentration were assessed using variance components analyses. ![]() The concentrations of multiple aliquots of the final dilution were measured by the OD, QG and PG methods. DNA concentration was estimated by OD and further diluted to 5 ng/uL. Twenty-Two lymphoblastoid cell line DNA samples with an initial concentration of ~350 ng/uL were diluted to 20 ng/uL. We evaluated the performance of spectrophotometric (OD) DNA quantification, and compared it to two fluorometric quantification methods, the PicoGreen ® assay (PG), and a novel real-time quantitative genomic PCR assay (QG) specific to a region at the human BRCA1 locus. The accuracy and precision of estimates of DNA concentration are critical factors for efficient use of DNA samples in high-throughput genotype and sequence analyses. ![]()
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